41. A sequence of in a genome at which replication is intiated in .........
A. origin of relpication
B. selectable marker
C. cloning site
D. origin of restriction
Answer : Option B
42. Genes which helps in the growth of transformants are .....
A. orgin of replication
B. cloning site
C. origin of restriction
D. selectable marker
Answer : Option A
43. Ti Plasmid is a cloning vector which works with
A. All the plants
B. Dicots only
C. Monocots only
D. Thallophytes only
Answer : Option B
44. During which of the following techniques host cells are exposed to pulse of highvoltage current ?
A. Electroporation
B. Particle Bombard ments
C. Micro injection
D. lipofection
Answer : Option A
45. Particle bombardment technique is also known as .....
46. Which enzyme is used to break the membrane to relase plant DNA ?
47. Which enzyne is used to break the membrane to relase animal DNA ?
48. Which is the first step in the process recombinant DNA technology ?
A. denaturing of DNA
B. Annealing of DNA
C. Isolation of Donor DNA
D. Down streaming
Answer : Option A
49. Which primers are used in annealing during amplification of gene ?
A. Reverse primers
B. Forward primers
C. Oligo nucleotide primer
D. Internal primers
Answer : Option A
50. What is temperature required for annealing of DNA molecule ?